Ka Hou (Raymond) Lao

3 Questions 12 Answers 0 Followers

Questions related from Ka Hou (Raymond) Lao

How can I use normalization value calculated from GeNorm for normalization of gene expression in qPCR?

Dear all, I want to ask how do you accurately use GeNorm for normalisation of the gene expression from qRT-PCR using ddCT method? I have used the standard curve method before that is quite...

18 February 2015 4,438 9 View

How to process hydrolysed genomic DNA for running in HPLC/MS?

Hi I have hydrolysed/digested some genomic DNA into single nucleosides and I am interested to see the amount of each individual nucleosides in the samples using LC/MS. However we have run into the...

19 November 2014 5,106 9 View

When 2′-Deoxyadenosine monohydrate is dissolved in water, is 2′-Deoxyadenosine released and exists as a standalone compound away from the H2O?

I am trying to use deoxyadenosine as nucleoside standard and I realized that only 2′-Deoxyadenosine monohydrate is on sale in Sigma and other companies, but not the standalone compound like...

06 June 2014 1,515 3 View