K. Karthik

6 Questions 15 Answers 0 Followers

Questions related from K. Karthik

Smearing in PCR- HN protein gene of mesogenic Newcastle disease virus (from field isolates )

Hi, We are using published primers for PCR amplification of HN gene of mesogenic NDV (Forward A TGG ACA GCG CAG TTA GCC AAG & Reverse GGT AGC CCA GTT AAT TTC CA)- 2090bp product size. We use...

05 June 2017 5,963 5 View

Yeast expressed protein confirmation- Two bands in SDS PAGE- one desired & other lower Kda- What may be the reason?

While running the secreted protein in methanol metabolism by my Pichia pastoris GS115 clone. I am getting two band one is higher (around 50 KDa) than the desired size (41 Kda by prokaryotic...

07 March 2017 2,655 7 View

Can a sample preserved in 50% glycerol saline be used for isolation of bacteria?

What will happen if a sample is send in glycerol saline 50% concentration. Why cant it be used for bacterial isolation.

15 September 2016 5,072 5 View

Suggest any broth that can be used for cultivation of Moraxella spp.?

Hi, I am having a Moraxella culture in blood agar and I wish to preserve it in glycerol stock. I tried to grow the culture in nutrient broth and also in trypitc soya broth but there was no growth...

30 March 2016 7,947 9 View

Any suggestion about culturing Clostridium chauvoei?

I am using RCM and ATCC 2107 media for culturing C. chauvoei but sevaral times I get mixed culture even while reviving from stock pure culture. for ATCC 2107 i am using anaerobic gas pack also....

31 March 2015 6,480 5 View

How can I find the toxin motif in a toxin gene?

Hi, I have a sequence of toxin genes of a bacteria. I would like to find out the motif that is toxic and if there is any bioinformatics approaches to it?

22 January 2015 7,230 8 View