Jyoti Motwani

3 Questions 5 Answers 0 Followers

Questions related from Jyoti Motwani

What special conditions are required for a smaller protein in western blot?

Hi I am trying to get protein expression of a protein called AVPI1. The size of this protein is quiet small-17kda.I have tried western blot for this protein twice- once with 20mcg of protein and...

02 February 2017 1,167 3 View

What should be the % of FBS added in the lower well in invasion assay?

Hi, What should be the % of FBS added in the lower well of the cell invasion assay? I added the complete medium in the lower well which was prepared by 5% FBS and 0.1% ITS as growth supplement. ...

09 September 2016 8,379 5 View

How can I tag the transfected cells?

I would like to induce the cells with FUCCI constructs then knock down the cells with siRNA for a particular gene .Then I would like to use those cells for 3D spheroid assay. To be able to confirm...

08 August 2016 9,589 1 View