Jyoti Singh

3 Questions 7 Answers 0 Followers

Questions related from Jyoti Singh

Can we combine relative abundances of OTUs derived from two different sequencing runs?

How can I best analyze the 16s amplicon sequencing data obtained from multiple runs? Do I have to run all the sequencing files in Qiime again? Are there any tutorials available?

29 October 2021 5,571 6 View

How to improve the purity of genomic DNA extracted from dust samples?

Hi I am extracting genomic DNA from dust samples and the 260/280 ratio is 1.4 whereas 260/230 is 1.35. I need to perform the metagenomic sequencing and for the same, the recommended 260/280 ratio...

23 April 2021 366 9 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

07 February 2021 2,821 4 View