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How I count a number of macrophage on coverslip in culture after differentate from monocyte?

I differentiated monocyte- derive macrophage on coverslip in 24 well plate with 1x10^6 cell/ml in total volume 1 ml per well but some cells died after seeding. I have washed non-adherent cells at...

03 July 2015 1,244 6 View

Can 1%BSA act as a carrier protein for storage of cytokines (GM-CSF, IL-4)?

Dear, I reconstituted the lyophilized rh IL-4 and GM-CSF (ImmouoTools ) with 1% BSA in sterile water (Data sheet recommend 0.1% BSA in sterile water) for long term storage and kept at -18 C...

07 June 2015 7,881 5 View