Junaid Asghar

3 Questions 6 Answers 0 Followers

Questions related from Junaid Asghar

How to calculate Emax percentage from GraphPad prism values?

How can I measure Emax percentage in a dose response curve when dealing with EC50 or IC50 values of my agonists from GraphPad prism data/ graphs?

21 June 2016 6,509 4 View

Why I am not getting a descent delta opioid agonist mediated inhibition of forskolin stimulated cAMP in SEAP assay?

I have a stably transfected cell line: CHO CRE-SEAP with GFP tagged delta opioid receptor, using dual selection antibiotics: hygromycin and G418. I did the conventional reporter gene assay (SEAP...

30 May 2016 9,198 3 View

My agonist stock is 10mM made in DMSO. How to go about with the vehicle control for a serial dilution starting from 10uM?

The question is basically about doing the vehicle control test for DMSO in mammalian cell culture (CHO cells). A working strength of 0.1 to 0.5 % DMSO is generally advised to be safer for most...

12 April 2016 3,477 5 View