Jun Xing

3 Questions 4 Answers 0 Followers

Questions related from Jun Xing

Which primer should I use if I want to detect the mRNA level of Akt in HCC cell lines?

Dear All, In my western blotting experiment I found a decrease level of both Akt and pAkt expression. So I want to check the mRNA level of them. I am wondering which primer should I buy for the...

17 October 2016 3,722 4 View

Soft agar staining reagent?

I want to staining the live cell colonies is soft agar assay. My lab we have INT(Iodonitrotetrazolium chloride) it's in solid state, the catalog is I8377 sigma. I want to know how to prepare it...

30 June 2016 1,670 3 View

Are protein ladders always precise? where is my target protein p27?

I want to use western blot to check the protein level of p27, I use the protein ladder from Thermo Fisher. and the p27 antibody from cell signalling. I cut the membrane between 55kDa to 35kDa...

01 January 1970 1,362 9 View