5 Questions 6 Answers 0 Followers
Questions related from Jun Xing
I did cell cycle assy with PI stained cells using FACS analysis and found my drug has the G1 block effect on cell cycle. The percentange of G1 phase increased from 63% to 78% and correspondingly...
23 March 2017 479 6 View
I am working on a new developed drug for its anti-tumor effect. Currently I am finishing the in vitro part with tumor cell line experiment. I have tested the IC50 in vitro. Now I want to inject my...
17 January 2017 1,396 4 View
Dear All, In my western blotting experiment I found a decrease level of both Akt and pAkt expression. So I want to check the mRNA level of them. I am wondering which primer should I buy for the...
17 October 2016 3,738 4 View
I want to staining the live cell colonies is soft agar assay. My lab we have INT(Iodonitrotetrazolium chloride) it's in solid state, the catalog is I8377 sigma. I want to know how to prepare it...
30 June 2016 1,673 3 View
I want to use western blot to check the protein level of p27, I use the protein ladder from Thermo Fisher. and the p27 antibody from cell signalling. I cut the membrane between 55kDa to 35kDa...
01 January 1970 1,364 9 View
