Julius Ryan Pronto

4 Questions 6 Answers 0 Followers

Questions related from Julius Ryan Pronto

How can I measure Indo-1 fluorescence using clampex?

I am using cardiomyocytes loaded with 5 uM Indo-1. I just want to know how I should set up my recording protocol if I want to measure dual emission from Indo-1. I am using 2 PMTs (both excited...

10 October 2018 394 5 View

Is it okay to store PFA-fixed cells long-term?

I'm working with cytospinned human cardiomyocytes and would like to stain several slides all at the same time. My current protocol makes use of 4% PFA with a permeabilization step combined with...

09 September 2018 3,198 3 View

What is the most reliable housekeeping gene for western blot analyses using sarcoplasmic reticulum vesicles?

My target proteins are RyR2 and SERCA2 and their respective regulators.

03 March 2016 2,560 4 View

Is there a special procedure for cardiomyocyte isolation for diabetic and aging rat hearts?

We have been trying to isolate cardiomyocytes from type 2 diabetes model OLETF rats using conventional procedure for adult rat hearts but we always fail from obtaining viable cells. The rats are...

11 November 2014 4,044 10 View