Julio Maia

5 Questions 12 Answers 0 Followers

Questions related from Julio Maia

How long can I store bacteria in LB to use it in a MiniPrep?

I was just wondering if it would be possible to store my bacteria after I grow them for a miniprep and what would be the best condition for it in case I just want to use them for a new DNA...

02 February 2016 5,911 4 View

Has someone already used TransTaq® DNA Polymerase High Fidelity for cDNA PCR amplification?

I would like to know if someone has used this Taq, TransTaq® DNA Polymerase High Fidelity, and if it compares with Phusion High fidelity. Thanks a lot!

28 January 2016 7,703 4 View

Any advice on a problem with PCR from plant cDNA samples?

Hi everybody, I am trying to clone a couple of plant genes and I am not getting amplification products when using cDNA. By luck, one of the genes I chose does not have introns, so I managed to PCR...

13 October 2015 5,837 9 View

What is the percentage of recalcitrant seeded species in different biomes?

Does anyone know of any other estimations besides the one by Walters et al, (2013), Science? (Walters, C., Berjak, P., Pammenter, N., Kennedy, K. and Raven, P. (2013) Preservation of recalcitrant...

17 October 2014 5,289 2 View

Why do seeds accumulate chlorophyll during development?

If seeds don't perform photosynthesis why do they accumulate chlorophyll? There is also a strong correlation between chlorophyll content and longevity of seeds during development.

07 May 2014 5,099 7 View