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Questions related from Juliette Bitard
Hi, I've encountered issues when fixating P12 mouse retinas with 4% PFA 30 minutes at room temperature. After the IF (24h 1ry antibodies in DAKO 0.3% Triton, 4 washings in PBS Triton 0.5%, 2ry...
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Hi everybody, Graphpad prism is able to calculate geometric means and geometric standard deviation. - Is there any requirement for the data to have a normal distribution in order to calculate the...
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Hi everybody, I’m trying to set-up the fixation/sonication experimental conditions for future ChIP-seq experimentq. I would appreciate to get some help for the analysis of sheared chromatin on...
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Hi everybody, My last post was about how to set-up the OIR model in the mouse. The present one is about the ethical statements to be taken into considerations. Does anybody have any legal...
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Hi everybody, Can anyone describe the exact protocol to set up this animal model of retinal degeneration? My questions are as simple as: (i) When you put back to normoxia the mice at P12...
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Verteporfin (SIGMA) is supposed to be dissolved 2mg/ml in DMSO according to the datasheet but I need to inject between 0.2 mg and 1mg per mouse. I can't inject 100% DMSO... And I'm working on 8g...
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Have you ever had issues in the reliability and efficiency of your intraperitoneal injection in mouse or rats? If yes you may probably as we did before in the lab (I'm sure we are not the only...
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