How to avoid non-specific binding in affinity chromatography (Ni- NTA resin)?

Hey, I need help! I'm trying to purify two proteins (26 kDa and 96 kDa) using a Ni- NTA resin. Both enzymes have a 6- histidine tag (bioinformatic models shows that the tags are correctly...

23 March 2023 3,901 10 View