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Does anybody have experience using the Qiagen gDNA eliminator with the aqueous solution of a trizol/chloroform RNA extraction? I see a lot of protocols with people using the RNeasy Kit after...
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Even though I use TBST 5% BSA for my blocking and phospho-antibody incubation, I get a lot of background (the film is completely black at the membrane location). I block for 2 hours at RT, primary...
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