Juliano Machado

5 Questions 7 Answers 0 Followers

Questions related from Juliano Machado

Does anyone have used 2% FBS to differentiate C2C12 myoblasts to myotube?

Hi everybody Does anyone have used 2% FBS to differentiate C2C12 myoblasts to myotube? If yes, Does 2% FBS work fine as 2% HS? Thanks a lot, Juliano

14 May 2018 9,739 2 View

Could mTORC1 dissociate the inhibition of autophagy from the stimulation of protein synthesis in skeletal muscle or any other cell type?

I have observed in my experimental model (skeletal muscle of mice and rats, C2C12 myotubes and primary cardiomyocytes cells) an activation of mTORC1 and inhibition of autophagy, however, no...

06 February 2018 6,730 0 View

Is transfection efficiency of C2C12 cells with Lipofectamine 2000 or 3000 better than PEI?

Is transfection efficiency of C2C12 cells with Lipofectamine 2000 or 3000 better than PEI? And how about Fugene?

16 July 2015 6,266 9 View

What is the correct manner to represent the effects of a treatment in autophagic flux assay in vitro?

Dear all, In an in vitro experiment of autophagic flux assay by western blot using chloroquine, is it correct to use the LC3-II/LC3-I ratio as a manner to represent the effects of a treatment or...

01 January 1970 5,344 2 View

How would you all interpret the following results of autophagic flux?

Dear all, How would you all interpret the following results? Please note the cells were incubated for 24 or 48h in conditioned medium in the presence of chloroquine during whole time-course of...

01 January 1970 3,687 4 View