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Questions related from Julia Mawer
When I transform linear PCR products that contain the KanMX4 cassette, I usually plate on YDP, incubate overnight, and then replica plate on G418. This works a charm. However, this time, I am...
18 March 2020 9,746 4 View
Hello, I have a very odd problem. A few years back (2016) I was using MyOne Streptavidin C1 dynabeads and coupling them to a biotinylated peptide and doing pulldowns using whole cell lysates. My...
10 October 2019 8,518 5 View
I am following standard protein precipitation. Mix 1:1 sample and 50% TCA for a final TCA of 25%. Ice 10 min then centrifuge 14K RPM 4 degrees 5 min and I see a lovely translucent pellet (30ug...
26 March 2019 4,046 3 View
I do not have a plasmid containing the NLS. I was planning to add the NLS to the sequence of a primer. My question is: Do I just place the sequence straight after the Start codon without a linker...
30 August 2016 832 4 View
I have a protocol for digesting yeast chromatin with MNase and when I run the DNA on a 1.5% agarose gel, the majority of my species are indeed in the mononucleosome form. However, I wish to do an...
12 May 2015 6,078 3 View
Does stripping with only glycine, SDS and tween tend to leave a background signal?
10 December 2014 2,939 4 View