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Questions related from Juhyun Chung
I am currently culturing SH-SY5Y cells, but recently, I've noticed unusual changes in their morphology. In the past, even after extended passaging, this kind of change never occurred. However,...
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I'm currently doing total cholesterol assay in SH-SY5Y cells treated with 0, 25, 50, 100ug cholesterol/mL (using cholesterol:mbcd, sigma) Lipid extraction is done by chloroform and methanol. After...
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I'm working on SH-SY5Y viability test with MTT assay, using vitamin C and H2O2. In our lab protocol, after 30-48h from cell seeding it reaches 70% confluency and gets ready for cell deprivation....
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HepG2 was fine until last Saturday(third picture), but today they detached and the color turned black...(first and second picture) Are they dead? or contaminated? After the subculture, I could...
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