Juhi Arora

5 Questions 6 Answers 0 Followers

Questions related from Juhi Arora

Seeking suggestions for replacing wax for PCRs? Does Mineral Oil work?

For our PCR reactions, we would add wax solution available from Biorad to prevent evaporation and inter-mixing of samples during the run. Since that product has been discontinued, we went around...

11 September 2020 3,591 2 View

What is the optimum time to add BFA or monensin while stimulating cells for cytokine production?

Working with murine spleenocytes. I stimulate 1 million cells with 50ng/ml PMA, 2ug/ml Ionomycin, 10ug/ml Briefeldin A for 4 hours at 37degC 5%CO2, followed by intracellular staining for...

02 September 2020 878 4 View

What is the best percoll gradient % to use for isolating lymphocytes from the lung?

I would like to isolate lymphocytes from the lung. One of the step requires a density gradient centrifugation step but most protocols make use of pre-made solutions like EasyColl. Has anyone used...

04 August 2020 7,399 3 View

We recently switched to a new PCR machine and all our old protocols stopped working. Any tips?

We have been trying to run genotyping protocols. We ran a gradient PCR to re-optimize the annealing temperature, tried to change the ramp speed and also modified the DNA purification protocol. Has...

20 July 2020 5,053 3 View

Has anyone used existing datasets of RNA-seq etc from published literature for extracting data?

Hello, I would like to utilize a published dataset of RNA-Seq for extracting information, such as upregulation/downregulation of transcripts of relevance to my project. The published dataset is...

19 March 2018 1,769 3 View