Judit Pénzes

4 Questions 6 Answers 0 Followers

Questions related from Judit Pénzes

Which is the best Lipofection agent on the market nowadays?

I have to order a new product to perform lipofection. So far I used Cellfectin II reagent from Invitrogen but not sure this one is actually the best one as well. I need to transfect insect cells...

11 November 2016 3,030 6 View

Sf9 cells won't transfect... Any suggestions on how to improve?

I am working with the bac-to-bac expression system and following the Invitrogen protocol available online. I have generated my recombinant bacmid and now I should transfect my cells, though they...

05 May 2016 7,871 2 View

SF9 cells suddenly start growing rapidly even though they are supposed to be infected... Has anyone experienced the phenomenon?

On a six-well culture dish there is one well, which is overcrowded with cells and this happened during a weekend (difference can be observed even without microscope - see picture attached). They...

12 December 2015 6,029 5 View

Phusion High-fidelity Polymearse: does anyone have experience with the GC buffer?

I am working on the amplification of a 3000-bp-long fragment from a viral genome wich has an average GC content of 45%. I have tryed several primers so far, and different adjustments with Takara...

06 June 2013 1,077 6 View