Juan Vaca

2 Questions 1 Answers 0 Followers

Questions related from Juan Vaca

What concentration of collagenase and hialuronidase are correct for extract cells from collagen-hyaluronic hydrogels?

Hi, I'm trying to extract cells from Collagen-HA hydrogels, but after enzymatic degradation of hydrogel with collagenase at 10 U/mL and hyaluronidase at 100 U/mL the cells that I extract are dead....

02 February 2018 7,536 2 View

I am trying to culture chrondrocytes in alginate, but cell death is 90% after counting. How can I culture cells and have viability at day 7?

I have followed several protocolos and even manufactures specifications (LONZA), but I have not obtained cell viability at the end of the assay. Moreover, cells are death by day three.

05 May 2017 7,910 0 View