Juan Minoia

3 Questions 3 Answers 0 Followers

Questions related from Juan Minoia

Does anyone have experience using M.I.O medium for E.coli growth and selection in cloning techniques?

Hi everyone, In our lab we're running out of LB ingredients and it's becoming hard to buy them. However, we do have M.I.O medium. Has anyone ever used this medium or another one specific for...

30 December 2023 367 2 View

DpnI digestion followed immediately by double digestion?

Hi, Did anyone tried DpnI digestion and immediately after that step double digestion with other two restriction enzymes? I did Clean Up after PCR and forgot to do DpnI digestion to get rid of...

11 November 2019 2,574 3 View

Does anyone have access to pGro7 sequence?

Hi, I need to know the sequence of pGro7 to check the transformation but I can't get the sequence on the internet. Neither can I request it to the distributor due to a collegue lent me a few...

14 July 2017 4,300 4 View