Juan Martinez-Olivan

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Questions related from Juan Martinez-Olivan

What instruments do yo use for homogenization and sonication of large E. colli pellets?

We are producing recombinant proteins in E.coli and we are interested in resuspension of 50-1000 gr pellets in an easier way than using a vortex or pippette so we look for homogenizators or other...

24 August 2020 650 5 View

How do you prepare isolated alpha and beta globins from commercial Hemoglobin (powder)?

I am looking for a protocol to obtain soluble and well folded alpha and beta globins. I saw some that are modifications of the Geraci protocol. Do you have any suggestions? Does alpha globin stays...

23 June 2016 9,751 5 View

It is more important to optimize DNA sequence in yeast or mammalian expression of proteins?

If you want to test the expression of a virus protein in mammalian cells (HEK) and yeasts (P. Pastoris) and you could only optimize the DNA sequence for one of the two cases, which one would you...

04 November 2015 4,194 1 View