J.P. Mandal

4 Questions 17 Answers 0 Followers

Questions related from J.P. Mandal

How to improve the transfection efficiency for CRISPR-mediated knockdown?

The CRISPR vector [GeneArt™ CRISPR Nuclease Vector] contain an OFP reporter but is not encoded with antibiotic resistance gene. 12-24 hours following lipofection, there was no significant orange...

06 June 2017 9,202 0 View

How can I immortalize fallopian tube epithelial cell without impairing P53?

Purpose is to study the impact of mutated p53 protein in tumorigenesis. hTERT alone isn't sufficient to immortalize human epithelial cells while other methods known to my knowledge turns down...

10 October 2016 362 3 View

Why did the immortalized cells not form colony in soft agar and did not produce tumor in NOD-SCID mice?

Primary fimbria epithelial cells were transduced with HPV16 E6/E7 and subsequently with lentiviral hTERT to generate immortal cell FE25. But this cell did not form a colony when grown in soft agar...

03 March 2016 7,626 2 View

What will be the path of the PCR reaction if I use a monovalent cation like k+ (using KCL) instead of MgCl2?

Mg2+ ions acts as cofactor for polymerase and it is also said that nucleotides which are added by this polymerase are also Mg2+ bound. Can k+ ions bind to the dNTPs or do they act as cofactor?

08 August 2013 730 5 View