Joydeep Roy

6 Questions 1 Answers 0 Followers

Questions related from Joydeep Roy

Are there cell culture based assays which separately assess the cytoplasmic or nuclear function after localization of the proteins ?

After performing some siRNA knockdown studies and overexpression studies I wanted to check my target protein localizing to the nucleus. After that I wanted to know the effect of localization on...

03 June 2023 1,046 0 View

Performing drug treatment and siRNA transfection together ?

Currently, I wanted to perform an experiment where I have to perform a drug treatment and siRNA transfection together. What shall be the way to design the protocol? Shall I perform the drug...

13 February 2023 6,822 2 View

How to get the gene knockout in AN3CA cell lines using CRISPR-Cas9 technology ?

Currently I work on endometrial cancer and I work on the gene PAK1. I had prepared knockout in endometrial cancer cells like Ishikawa but when it come to making knockout cells in AN3CA I have a...

29 November 2022 6,192 3 View

Scratch assay in siRNA transfected cells ?

I work with siRNA transfection in endometrial cancer cells where 72 hours after siRNA transfection, the cells reach a maximum of 65% to 70% confluency. In contrast, 95% to 100% confluency is...

01 January 1970 4,028 1 View

How to generate an mdp file for gromacs ?

I wanted to perform a molecular dynamics simulation for a kinase and wanted to know how to generate mdp files for adding ions after solvation ? I could successfully generate the topol.top files...

01 January 1970 2,128 4 View

How to design primers for cloning if I need to have a fluorescence tag in the molecule in C terminal ?

I need to clone a gene (2KB sequence) from one plasmid to some other plasmid which needs to have a fluorescence tag. After proper sequencing, I could find there is presence of the stop sequence...

01 January 1970 7,922 3 View