Joy Das

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Questions related from Joy Das

How to inhibit non-enzymatic hydrolysis of acid labile unstable substrates (like ATP, UTP etc) during inorganic phosphate detection?

Detection of inorganic phosphate using Malachite green assay is usually associated with two crucial problems hampering colourimetric assays: 1) Precipitation of end product 2) Hydrolysis of...

17 June 2020 9,253 0 View

How to calculate V0 (initial velocity) for a discontinuous enzymeaic assay?

Usually, for fixed-time or discontinuous enzyme assay, a specific incubation period (say 30 minutes incubation period) is required before the reaction is stopped and measured (say...

01 May 2020 2,684 2 View