Josip Madunic

3 Questions 3 Answers 0 Followers

Questions related from Josip Madunic

How stable is HRP on secondary antibody in western blot?

Hi all, I have been having problems with my blots recently which I cannot explain logically so I have a few questions about the basics of Western blot and hoping your answers can shed some light...

19 March 2015 4,263 4 View

Positive control for phospho-ERK in MDA MB-231 cells?

Does anyone know a good positive control (treatment) for ERK activation in MDA MB-231 cells? Thank you in advance.

10 March 2015 1,562 8 View

Does anyone have experience in working with human glioblastoma cell line A1235 and knows its mutations?

The A1235 cell line is known to be deficient in repairing alkylating damage due to missing an activity of MGMT enzyme, crucial in repairing alkylating damage. I am having a tough time finding...

09 December 2014 7,452 2 View