Josephine Swiggs-Malmevik

4 Questions 10 Answers 0 Followers

Questions related from Josephine Swiggs-Malmevik

How do I quickly find out whether an AAV plasmid is single-stranded or self-complementary?

Hi all, We have purchased an Addgene plasmid (#61593) and cannot find out in articles referencing it (have not emailed the authors yet) whether this is an ss or sc vector. What is the quickest...

19 October 2016 6,360 2 View

How much would glycosylation affect the size of a 122kDa protein?

How much would you estimate the the final product of a 122 kDa protein is after glycosylation? (Predicted to have 67 O-linked glycosylation sites + 8 N-linked glycosylation sites). N-linked...

13 January 2016 3,057 7 View

Can anyone recommend a specific antibody and/or target to co-localise a protein in the secretory pathway?

Would anyone recommend a specific antibody and/or target to co-localise a protein in the secretory pathway? Would you recommend targeting the rough endoplasmic reticulum, if so which antibody (as...

13 November 2015 1,896 5 View

Can anyone help with AAV transduction in SH-SY5Y cells?

I have some questions about using AAV in SH-SY5Y cultures: - What kind of MOI / # genome copies per ml or similar would you start out with to test in cultures? I plan to use SH-SY5Y...

07 August 2015 6,083 10 View