Joseph Valentino Raimondo

3 Questions 7 Answers 0 Followers

Questions related from Joseph Valentino Raimondo

Does anyone have experience with an alternative to the Biorad Helios Gene Gun system for biolistic transfection?

I am hoping to perform biolistic transfection of organotypic brain slice cultures. In my previous lab we used the Biorad Helios Gene Gun System - which effectively is just a pressurized gun that...

06 June 2018 3,851 3 View

Are there opensource alternatives to Neurolucida for neuron tracing and reconstruction?

We are looking to reconstruct biocytin filled neurons from confocal image stacks. I realise Neurolucida is the gold standard but even the Neurolucida 360 lite version is hideously expensive (~$15...

03 March 2018 5,310 11 View

Why do we experience pipette blockages/ clogging with our potassium gluconate based internal solution?

We are attempting multi-patch clamp recordings in cerebral cortex but are experiencing severe blockage issues. Our pipettes are frequently clogging. Our internal contains: K-gluconate (120...

12 December 2014 8,064 16 View