Why do we use 10X SPG buffer (2.18M sucrose; 0.038M KH2PO4; 0.072M K2HPO4; 0.049M Na-Glutamate) when freezing virus stocks?

Further, how does one calculate the pH of such a buffer solution? Using a pH meter is impossible due to the high viscosity of the buffer solution... Any chemists here?

08 March 2024 5,220 1 View