Jose Sofia Ruffino

4 Questions 21 Answers 0 Followers

Questions related from Jose Sofia Ruffino

Could anyone recommend an amplex red assay or acconitase assay kit for use with human muscle samples?

We are analysing NADPH production and oxidative damage in human vastus lateralis muscle and flitting between the idea of using 'homemade' kits or commercial kits for these analyses. Has anyone...

06 June 2015 6,740 0 View

Are there any human muscle biopsies for Western blotting?

I am currently using human vastis lateralus samples taken using a Bergstrom needle. The samples are currently stored in liquid nitrogen. I am hoping to use these samples for Western blots, looking...

07 July 2014 7,925 4 View

RNA extraction from muscle - low 260/230 ratio - any thoughts?

I have extracted RNA from human muscle biopsy samples (~10-20mg starting material) using TRIzol and the chloroform/phenol method of extraction for use in RT-qPCR (SYBR green method). The samples...

07 July 2014 7,990 6 View

Protein lysis and storage for Western Blot: Is boiling necessary prior to storage?

I am currently in the process of propagating primary human macrophages for protein samples to be used in Western Blot. With cultured cells I have used the RIPA buffer method (RIPA buffer plus...

01 January 2013 9,801 26 View