Jose Manuel Latorre Estivalis

9 Questions 29 Answers 0 Followers

Questions related from Jose Manuel Latorre Estivalis

Does anyone has experience with CummeRbund package to analysis RNAseq data?

I am analyzing Tophat-Cufflink-Cuffdiff out put with CummeRbund package in R. I have read the CummeRbund manual and Tramper et al., (2012) papers about this program, however, I still have problems...

02 February 2015 9,203 2 View

How can I measure gene expression of a gene with a single exon using qPCR?

I would like to measure the gene expression of a target gene in different tissues by means of qPCR. However, this gene has a single exon and I don't know how I can distinguish between gDNA and...

01 January 2015 6,067 8 View

Has anyone normalized data from RNAseq using BWA software?

I have performed an analysis of my RNAseq data using BWA software. The final output of this analysis is number of reads per target gene. I would like to compare these data with my qPCR data,...

11 November 2014 6,785 11 View

How do you analyse Bestkeeper output?

I am working with Bestkeeper to select my reference genes. I am trying to find a criteria to classify Bestkeeper output (from most stable to least stable gene). I have read Pfaffl paper...

01 January 2014 8,586 31 View

Any suggestions for RNA extraction of tissues with high chitin content?

I am making RNA extractions with insect antennae and I would like to increase the yield of this process. For this, I use Trizol and I disrupt tissues manually with a pestle. Other disruption...

04 April 2013 5,406 11 View

Relationship between Ct values of target and reference genes in qPCR.

I am doing PCR experiments with different target genes and different potential reference genes (housekeeping genes). I have read that to apply Delta Delta Ct method, the Ct value of reference...

04 April 2013 5,980 7 View

Information about SuperScript™ III (Invitrogen).

I will start using SuperScript™ III to make RT reactions. I have some doubts about the ideal temperature in RT reaction and about the amount of enzyme per reaction. In the protocol, they suggest...

03 March 2013 4,268 4 View

Anyone know a good phylogenetic method for genetic population analysis?

I have sequenced several molecular markers (ITS-2, 18S and 28s rRNA genes and two mitochondrial genes, cox1 and cox2) from different mosquito species and populations. I would like to analyze the...

02 February 2013 1,992 10 View

Has anyone participated in the qPCR courses from TATAA Biocenter?

I would like to propose to my institute the possibility of contracting these courses, but they are really expensive. So, I would like to have some data or some opinions about them.

02 February 2013 6,784 6 View