José Pablo Romero-López

4 Questions 8 Answers 0 Followers

Questions related from José Pablo Romero-López

Have somebody tried to freeze fixed samples?

I need to analyze some tissue samples by FACS, but the analysis have to be performed after several months after the sample is taken and I have noted that when freezing cells with 10% DMSO in FBS...

25 October 2017 6,639 4 View

How can I demonstrate the induction of monocyte-derived dendritic cells?

Is there one specific marker just to validate the conversion to dendritic cells? I have found several panels to analyze the immunophenotype of DC's, but I would like to know if there is an option...

28 February 2017 8,245 4 View

Does anyone have expertice immortalizing human cells?

I am trying to immortaliza antigen presenting cells that express a specific HLA molecule, but I don't know what would be the best approach, a virus immortalization or protocols like hTERT, and...

19 September 2016 6,665 1 View

Can I stimulate PBMC's with PMA/Ionomycin for 4 hours and then wash them, incubate them on medium and stain them 15-24 hours after that?

I need determine cytokine production on T cells but it is hard for me to do the whole procedure on the same day

21 April 2015 6,137 8 View