Jose Maria Aguilar-Camacho

7 Questions 1 Answers 0 Followers

Questions related from Jose Maria Aguilar-Camacho

Dilution of a recombinant protein and its injection in live animals?

We ordered a recombinant protein from a famous biotech company. This recombinant protein penetrates a particular organ and we wanted to test if that molecular process occurs in this non-model...

02 March 2025 8,449 3 View

ISH and qPCR does not match in a gene KD?

I carried out an experiment in which I fed marine larvae with bacteria expressing dsRNA of a particular gene under different treatments and timepoints. I extracted RNA for qPCR and fixed samples...

07 July 2024 359 1 View

Any protocol for loading dsRNA in an agarose gel?

I am expressing dsRNA using E.coli as vector. I would like to check that my bacteria culture is expressing dsRNA. I am planning to extract RNA from my bacteria culture and then load the total RNA...

19 March 2024 1,400 3 View

Is there a method to stain ingested bacteria?

I am working with a marine non-model organism that has a planktrotrophic larval type and I found out that the larvae are not very selective while feeding. I am planning to do RNAi by feeding them...

26 January 2024 7,712 3 View

Is it possible to analyze single nuclei RNA seq libraries with an assembled transcriptome or only with the genome?

I am trying to generate single nuclei RNA seq libraries from a non-model organism. We have a genome that it is not well annotated and a bit incomplete. I know that for single cell RNA seq is...

03 October 2023 5,213 1 View

Any good protocol to refold recombinant proteins from inclusion bodies?

I am working on the expression of one protein with a MW of 22 KDA. This protein has 8 cysteines, pI: 8.58 and positively charged. This protein forms incusion bodies, I have done washes of the...

06 December 2017 7,025 2 View

What are the techniques to determine transcription binding sites (transcription start sites) in the genome of a non-model organism?

I am working with a non-model organism and we generated a complete genome that is annotated. How can you determine the transcriptions start sites in this genome? Is there any bioinformatic way to...

01 January 1970 9,554 1 View