Jordan Valdez

3 Questions 1 Answers 0 Followers

Questions related from Jordan Valdez

What is a good total bacteria staining alternative to Syto 9 to avoid bleeding into PE?

I'm doing IgA-seq, and we use anti-IgA conjugated to PE to bind endogenous IgA-coated bacteria in stool samples. Syto 9 bleeds into our PE channel pretty significantly on the CytoFLEX, and I...

20 June 2025 4,586 0 View

Trouble with MiSeq v2 Cluster Density?

I am having difficulty figuring out why my cluster density is consistently too high for my 16S sequencing runs on the MiSeq (v2 chemistry). I have been getting a cluster density between 1100-1300,...

14 February 2025 2,847 0 View

Completely wrong plasmid sequence in transformants?

I have transformed NEB 5-alpha cells with a plasmid that I have pre-sequenced and confirmed it has the correct sequence. All I want to do is make more of this plasmid. However, when I transform...

05 December 2023 8,208 3 View