2 Questions 8 Answers 0 Followers
Questions related from Jonathan Elegheert
Dear all, In lentiviral plasmids, the WPRE is usually placed near the 3'LTR, in the 3'UTR of the transgene. My question is; can WPRE be placed in the antisense direction, so near the 5'LTR,...
05 May 2019 6,223 5 View
Dear all, I am using a plasmid with following organization; 5'-insert-IRES2-GFP-3' (i.e. bicistronic vector). My question; how does expression of the GFP correlate with (or influences) the...
12 December 2017 8,288 10 View
