Jonathan Harold Sogin

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Questions related from Jonathan Harold Sogin

How do you design heterogeneity spacers for primers in amplicon metagenomic studies?

Adding heterogeneity spacers to primers used for Illumina based amplicon studies has proven to improve read quality by increasing bp diversity on the flow cell during sequencing. I have seen two...

02 November 2020 9,340 3 View

High throughput DNA extraction for yeast and bacteria from fermented beverages (wine, kombucha, non-dairy)?

Can anyone recommend a DNA extraction method, preferably without the use of a kit, that reliably extracts 'total' yeast and bacterial DNA from non-dairy fermented beverages? The beverages in...

12 July 2019 8,268 1 View