Jonathan Good

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Questions related from Jonathan Good

Conflict in DNA measurment readout between a TapeStation 4150 and a Qubit 4?

We regularly use both a Qubit 4 fluorometer and the TapeStation 4150 to measure DNA concentrations. We follow the recommended procedures for both devices but the TapeStation consistently gives the...

26 May 2023 7,659 0 View

Any suggestions to improve DNA library concentration even though I have good genomic DNA concentration?

I have been using the Qiagen Blood mini kit to extract DNA from 250 uL of bone marrow blood. I eluted the DNA in either TE or AE buffer because I thought EDTA concentration might be interfering...

09 February 2023 4,357 6 View

Any protocols or suggestions for maximizing DNA extraction from frozen bone marrow monocyte pellet?

I have frozen cell pellets of bone marrow monocytes. It is crucial to extract as much DNA as possible from them for sequencing. The pellets will have around 500,000 cells. I have used the QIAamp...

01 December 2022 1,111 1 View