John Sullivan

3 Questions 4 Answers 0 Followers

Questions related from John Sullivan

What are the best activation markers for T cells in human lungs?

Our lab is looking to buy antibodies that are markers for activated T cells in human lung tissue. Has anyone tried something like this before? What did you use?

02 February 2020 7,024 2 View

Has anybody successfully done Bisulfite sequencing on DNA extracted from Laser Capture Microdissection?

I am attempting to do bisulfite sequencing on a frozen lung section extracted by laser capture microdissection. So far, I have not had good results getting past the PCR amplification step. To...

10 October 2019 6,895 6 View

How does Laser Capture Microdiessection affect integrity of DNA?

I am capturing B cells from Lung tissue using LCM, and then isolating the DNA for various downstream assays. When I finish DNA isolation using the Qiamp mini kit, I am getting good quantity and...

09 September 2019 5,366 3 View