John Litchfield

4 Questions 3 Answers 0 Followers

Questions related from John Litchfield

How much ripa buffer do I add per mg of rat brain tissue?

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.

02 February 2016 7,966 0 View

What is the difference between 4% and 2% paraformaldehyde fixation?

What % of paraformaldehyde is better for fixing rat brain tissue for IHC?

09 September 2015 7,526 2 View

I am unable to obtain an occludin signal via WB on my rat brain samples. Is there any specific protocol to detect occludin?

Rat hippocampus homogenized in RIPA buffer with P/P inhibitors. BCA. Heated 70 degrees for 10 min. Run on 4-12% bis-tris gel with MOPS buffer. 7 Minute xfer with trans-blot turbo. Occludin...

01 January 1970 6,301 3 View

I am unable to obtain an occludin signal via WB on my rat brain samples. Is there any specific protocol to detect occludin?

Rat hippocampus homogenized in RIPA buffer with P/P inhibitors. BCA. Heated 70 degrees for 10 min. Run on 4-12% bis-tris gel with MOPS buffer. 7 Minute xfer with trans-blot turbo. Occludin...

01 January 1970 9,900 3 View