Lots of impurities after Ni_NTA purification of his-tagged protein?

I am trying to purify 50 kDa recombinant protein with his6-tag on the c-terminal by using Ni-NTA chromatography. I am using 1 mL HisTrap columns, and I loaded my sample (E. coli lysate containing...

22 May 2024 4,444 5 View