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Questions related from Johannes Petrus Louw
Hi everyone, I'm firstly interested in identifying Alternaria cultures to species level. After this, I will have a look at a specific pathotype. In this regard, would it be sufficient to use...
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Hi everyone, I'm looking for a relatively simple viability test method to distinguish between live and dead spores (fungi i.e. Botrytis or Penicillium). I need to be able to do this using a...
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Hi, We found what seems to be downy mildew on a native crop. Since this is not exactly my field of study, how do you go about isolating, purifying and identifying a biotrophic pathogen? We would...
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I'm working on Penicillium spp. that cause postharvest rot of stone fruit. The project has a gene expression segment where I need to quantify several genes of the pathogen from infected fruit (in...
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In a resent submission to a journal a reviewer commented: "As far as I know, viruses are self-replicating structures, using for this purpose nucleic acids identical in function to those present...
01 January 1970 1,534 15 View
Based on a resent question; what is the difference between a saprophyte and a saprotroph? In Greek saprophyte would be saprós (“putrid; decayed; rotten”) and phyte ("plant") thus meaning a...
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Hi, I'm starting with a gene expression study using droplet digital PCR (ddPCR). With qPCR a primer efficiency test is needed and MIQE guidelines for digital PCR (dPCR) seems to indicate that you...
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