Joel Christie

3 Questions 7 Answers 0 Followers

Questions related from Joel Christie

Can I use high pH reverse phase peptide fractionation kit along with iTRAQ reagents?

Hi all, This is regarding using Reverse Phase High pH Fractionation Cartridges being provided by Thermo. We are planning to use these cartridges for Fractionation before submitting iTRAQ...

05 May 2018 1,270 3 View

What should be the basis for deciding threshold cut off for Differential Expression of any given Proteomics Data?

Most of us will agree to a point that at protein level changes wouldn't be having greater differences like one may get at mRNA levels. Mostly in many a cases arbitrarily I have seen people...

07 July 2017 2,981 2 View

How to identify retention time of any particular peptide in any given LC MS run?

I want to quantify certain proteins using label free peptide peak intensity based method; for this I feel first of all I should know the retention time of identified peptides, and after...

05 May 2016 5,197 4 View