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I am trying to create stable GFP CHO cells using G418 as a selection agent. I carried out a dose response curve and determined 0.6 mg/ml would completely kill cells by day 7. I used 0.8 mg/ml for...
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I am looking to quantify plasmid integration very soon after transfection into mammalian cells. Therefore I need a qPCR method that can distinguish between integrated and extrachromosomal plasmid....
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