João Anes

3 Questions 3 Answers 0 Followers

Questions related from João Anes

What is the best RNA extraction method for Klebsiella pneumoniae?

I'm trying to isolate RNA for RNA-seq but it's hard to extract good quality RNA for Klebsiella pnuemoniae. I've tried TRizol and Direct-zol™ RNA MiniPrep but the quality of the RNA it's always low...

06 December 2015 809 2 View

Is there any enzyme mixture that I could use for the detachment of biofilm?

I’m testing the MBEC in Klebsiella pneumoniae and I would like to detach my biofilm to get CFUs counts. I would like to use an enzymatic method for degradation of the matrix. From the literature I...

10 March 2015 1,732 3 View

What is the best knockout strategy to generate Klebsiella pneumoniae mutants?

I'm currently working on efflux pump inhibitors and I would like to generate some Klebsiella pneumoniae mutants in my clinical isolates. However my expertise level is minimal. Could you give me...

20 February 2015 452 6 View