Does anyone have an optimised, working immunofluorescence staining protocol for BrdU?

In brief, I have been labeling my cells with 50uM BrdU over >2X the doubling time of the cells. Thereafter, I would wash the cells 4X with 1XPBS before fixing them with 4%PFA. The cells are...

11 April 2018 2,360 2 View