Joanna Klim

4 Questions 4 Answers 0 Followers

Questions related from Joanna Klim

How to desing “splinkerette” adapters for TraDIS (transposone) libraries?

I'm trying to optimize TraDIS for our model bacterium and I am having some technical problems with the DNA preparation for the sequencing step. I'm struggling with efficient PCR enrichment of...

13 September 2023 5,809 0 View

Is there any easy way to check if given miRNA hybridize to RNA (extracellularly)?

Suppose we have given miRNA (that according to in silico analysis should bind to a specific sequence in RNA) that is labeled with a fluorescent dye (or is labeled otherwise) and we have isolated...

13 November 2020 5,326 1 View

How to get rid of redundant GO terms?

I've got microarray transcriptomic data (from S. cerevisiae) - and I've made GO enrichment analyses - since there were many redundant GO terms I've used REVIGO for getting rid of them but I found...

03 September 2019 4,239 6 View

What is the best method to isolate/extract yeast DNA for genome sequencing purposes?

I would like to obtain high-quality DNA. There are a lot of protocols for DNA isolation but most of them are for PCR purpose and they are not appropriate for sequencing due to low quality. Thanks...

13 January 2016 9,695 6 View