Joan Martí

2 Questions 4 Answers 0 Followers

Questions related from Joan Martí

Best conditions to mantain epithelial phenotype in A549 cell line and avoid EMT?

Hi everyone. I am culturing A549 in F12K suplemented with 10% FBS and 1% PS. After staining cells mark very positive for SMA and almost no E-cadherin without the addition of TGF-B1. Why is this...

01 October 2020 4,220 0 View

Can anyone help with EDC/NHS in non-aqueous medium?

Hello, I'm trying the EDC/NHS method to link APTES on partial hydrolized-PLA nanofibers. I cannot use water as a solvent because PLA is hydrophobic and has a poor wettability. Instead I use...

11 August 2015 5,629 9 View