Jj Narayan

6 Questions 2 Answers 0 Followers

Questions related from Jj Narayan

What enzymes are in the Second Strand Synthesis/A-Tailing Enzyme mix in KAPA Roche RNA-seq Kit ?

In this kit: https://rochesequencingstore.com/wp-content/uploads/2017/10/KAPA-RiboErase-KitHMR_KR1142-–-v4.19.pdf What enzymes are used in the "Second Strand Synthesis and A-Tailing" enzyme...

11 June 2022 3,350 0 View

Fastest way to sequence a plasmid - should I use MiniSeq?

A lab that works with ours does pretty frequent MiniSeq runs. I was going to sequence this 12 kb plasmid (which we have almost no information about) by Sanger sequencing, then ordering new primers...

01 October 2021 2,788 2 View

Will low 260/230 (nanodrop) after cleanup affect ligation?

I digested and dephosphorylated a vector, and digested a PCR amplicon (my insert). Since I had to use a column to cleanup my vector digest before ligation (since CIP is not fully...

08 August 2020 3,836 1 View

Adding more isopropanol to eluted DNA in plasmid extraction?

In the Plasmid Mini Extraction kit by Qiagen, they say to elute the DNA with 800 uL of the elution buffer, then to add 560 uL room temperature isopropanol, and finally to centrifuge it for 30...

11 November 2018 9,221 3 View

What's the difference between the Qiagen Spin Miniprep Kit, and the Qiagen Plasmid Mini kit?

I'm an undergrad molecular biology student and I'm trying to extract a plasmid to use as a vector. I'm going to amplify my gene of interest (from a different plasmid in a different strain) using...

11 November 2018 856 4 View

Adding more isopropanol to eluted DNA in plasmid extraction?

In the Plasmid Mini Extraction kit by Qiagen, they say to elute the DNA with 800 uL of the elution buffer, then to add 560 uL room temperature isopropanol, and finally to centrifuge it for 30...

09 November 2018 4,770 3 View