Jiyoon Kim

3 Questions 1 Answers 0 Followers

Questions related from Jiyoon Kim

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

17 July 2024 7,692 1 View

How do you perform genotyping to check for the presence of loxP sites in a loxP mouse?

When crossing loxP mice with FLPe mice, LacZ and Neo are removed; how do you perform genotyping in this case? Additionally, can you determine if a loxP mouse is heterozygous or homozygous through...

17 July 2024 505 1 View

Can I label an antibody which is conjugated to FITC with FITC labeling kit?

I have a primary antibody conjugated with FITC, but the signal is too weak during flow cytometry. Would it be possible to use an FITC labeling kit to relabel the antibody and enhance the signal?...

01 January 1970 4,497 2 View