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I have been doing long-term cultures of primary cortical neurons for several years, from 2018-2021, with no problem. I use Neurobasal medium with 2% B27, 1% glutamax, 1% penstrep. I would grow my...
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I have read papers that describe methods for long-term primary neuron culture but I have not seen convincing western blots showing consistent changes in any protein expression with the progress...
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