Jiyeon Baek

4 Questions 4 Answers 0 Followers

Questions related from Jiyeon Baek

Why are my primary cortical neurons suddenly dying in culture?

I have been doing long-term cultures of primary cortical neurons for several years, from 2018-2021, with no problem. I use Neurobasal medium with 2% B27, 1% glutamax, 1% penstrep. I would grow my...

29 September 2022 3,820 2 View

Why does my methylene blue stain look normal, but my antibody stain cause halos in my dot blot?

In the attached images, the one that has even staining is the membrane stained with methylene blue, and the one with uneven staining is the antibody-probed membrane exposed on film. What could be...

17 December 2019 2,054 0 View

What are some aging biomarkers I can probe for in my long-term primary cortical neuron culture?

I have read papers that describe methods for long-term primary neuron culture but I have not seen convincing western blots showing consistent changes in any protein expression with the progress...

29 January 2019 9,191 3 View

How can I improve my protocol for differentiating iPSCs into astrocytes with serum?

I have been using 20% FBS in DMEM with L-Glu and NEAA. I started off with half feeds, and then eventually transferred the surviving cells to plastic to select for astrocytes. However, they did...

03 May 2018 9,257 3 View