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As we known, the naive B cell activation depend on T cell. The antigen alone cannot trigger B cell differentiation. However, whether the memory B cell could be activated by antigen without Th cell...
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I used to think that signal peptides were necessary for a membrane protein to be displayed on the cell surface till I found that a GPR with multiple transmembrane structures have not a signal...
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Recently, I want to modified the T cells with eGFP regulated by the NFAT signalling. And the attached is the map of the lentivirus plasmid. However, there is no fluorescence in T cells activated...
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