Jiexi Hou

2 Questions 15 Answers 0 Followers

Questions related from Jiexi Hou

Troubleshooting if the 35S promoter used in Hygromycin selectable marker gene of pMDC110 vector affects the expression of my transgene?

I cloned a phloem parenchyma-tissue specific promoter into pMDC110 vector (contains GFP) and introduced this clone into Arabidopsis. However, GFP signal was ectopically detected in all tissues of...

12 August 2015 3,313 14 View

Is there a good blunting enzyme to blunt 3' overhangs?

What I am doing is pretty straight forward. I am cutting out a fragment with two restriction enzymes. Then enzymes are needed to chew away the two 3' overhangs so I can circulate my plasmid...

16 April 2015 5,334 11 View